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Real Name: Quentin Beck
First Appearance: The Menace Of Mysterio
Voice: Greg Berger
Mysterio impersonated Spider-Man and committed crimes, bashing the wall crawlers less than sterling reputation in the process. His hatred for Spider-Man began when Spider-Man stopped Beck's plans to sabotage a film set, and was arrested for doing so. He swore revenge on the wallcrawler.

Upon his release, he used his skills as a special FX artist and stuntman to create artifical ways to duplicate Spider-Man's powers. With his 'powers' he began to commit robberies to fund his criminal activites and ruin Spider-Man and eventually kill him, just like in the movies. After his efforts failed, he was released from prison by Kingpin to join The Insidious Six.

After escaping again, he returned to Wonder Studios to gather his equipment. He discovered Miranda Wilson near death, and hooked her mind to machinary in order to keep her alive. He began to commit robberies to pay for the machinary until Spider-Man stopped him and Miranda realised that her dream to have her mind transported into someone else' body was a sham.

After Mysterio confessed his love for her, she blew Wonder Studios up with them inside it. Neither of them were seen again.