Aunt May
 

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Voice: Linda Gary
First Appearance: Night Of The Lizard

Aunt May and Uncle Ben raised Peter after his parents died. Uncle Ben was killed because Peter refused to stop a thief, leaving the 2 of them alone. Aunt May was forever dotting on Peter, loving him as if she was her own son. May tried numerous times to set Peter up with her friend Anna Watson's niece, Mary Jane. May was unaware that Peter was Spider-Man, and felt a strong hatred for Spider-Man.